Recombinant:Article Title: Circadian synchronization differentially modifies cytokine-mediated transcriptomic remodeling and cell death in INS-1 cells and mouse islets
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rat: INS-1 Gift from C. Wollheim and P. Maechler, University Medical Centre, Geneva, Switzerland RRID: CVCL_0352 Experimental models: Organisms/strains Mouse: C57Bl/6J Charles River Laboratories RRID: IMSR_CRL:027 Mouse: C57BL/6J mPer2:Luc The Jackson Laboratory RRID: IMSR_JAX:006852 Oligonucleotides For primers see Table S3 TAG Copenhagen N/A Recombinant DNA pPer2:Luc Gift from Prof. Louis Ptá�cek, University of California San Francisco N/A psPAX2 N/A RRID: Addgene_12260 pMD2.G N/A RRID: Addgene_12259 Software and algorithms AzureSpot Pro Azure Biosystems https://azurebiosystems.com/ software/azurespotpro/ BioDare2 Zieli�nski et al.59 https://biodare2.ed.ac.uk/ GraphPad Prism 9/10 GraphPad Software https://www.graphpad.com/ Image Lab 6.1 Bio-Rad Laboratories https://www.bio-rad.com/product/ image-lab-software?ID=KRE6P5E8Z Improved Proteasome Cleavage Prediction Server (iPCPS) Gomez-Perosanz et al.60 http://imed.med.ucm.es/Tools/pcps/ QuantStudioTM Design & Analysis Software Applied Biosystems https://www.thermofisher.com/home/ technical-resources/software-downloads/ quantstudio-3-5-real-time-pcr-systems.html Other Azure®Saphire Biomolecular Imager Azure Biosystems N/A CLARIOstar Plus with Atmospheric Control Unit (ACU) BMG LABTECH N/A Histopaque®-1077 Hybri-MaxTM Sigma-Aldrich Cat# H8889 Histopaque®-1119 Sigma-Aldrich Cat# 11191 iBLOT2 system Thermo Fisher Scientific (InvitrogenTM) N/A iBlotTM 2 Transfer Stacks, PVDF, mini Thermo Fisher Scientific (InvitrogenTM) Cat# IB24002 LumiCycle Actimetrics N/A NuPAGETM Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm Thermo Fisher Scientific (InvitrogenTM) Cat# NP0323BOX QuantStudio5TM Applied Biosystems N/A Radiance Q Chemiluminescent Substrate Azure Biosystems Cat# AC2101 SpectraMax® i3x Molecular Devices N/A iScience 28, 112431, May 16, 2025 e2 Pancreatic islets for circadian bioluminescence recordings were isolated from adult (approximately 15 weeks old) female C57BL/ 6J mice expressing the circadian reporter Per2-luciferase (mPer2:Luc) (The Jackson Laboratory, Bar Harbor, ME, USA).61 Mice were kept under standard temperature-controlled animal housing conditions with ad libitum access to food and water and 12h:12h lightdark cycle. ..
Software:Article Title: Circadian synchronization differentially modifies cytokine-mediated transcriptomic remodeling and cell death in INS-1 cells and mouse islets
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rat: INS-1 Gift from C. Wollheim and P. Maechler, University Medical Centre, Geneva, Switzerland RRID: CVCL_0352 Experimental models: Organisms/strains Mouse: C57Bl/6J Charles River Laboratories RRID: IMSR_CRL:027 Mouse: C57BL/6J mPer2:Luc The Jackson Laboratory RRID: IMSR_JAX:006852 Oligonucleotides For primers see Table S3 TAG Copenhagen N/A Recombinant DNA pPer2:Luc Gift from Prof. Louis Ptá�cek, University of California San Francisco N/A psPAX2 N/A RRID: Addgene_12260 pMD2.G N/A RRID: Addgene_12259 Software and algorithms AzureSpot Pro Azure Biosystems https://azurebiosystems.com/ software/azurespotpro/ BioDare2 Zieli�nski et al.59 https://biodare2.ed.ac.uk/ GraphPad Prism 9/10 GraphPad Software https://www.graphpad.com/ Image Lab 6.1 Bio-Rad Laboratories https://www.bio-rad.com/product/ image-lab-software?ID=KRE6P5E8Z Improved Proteasome Cleavage Prediction Server (iPCPS) Gomez-Perosanz et al.60 http://imed.med.ucm.es/Tools/pcps/ QuantStudioTM Design & Analysis Software Applied Biosystems https://www.thermofisher.com/home/ technical-resources/software-downloads/ quantstudio-3-5-real-time-pcr-systems.html Other Azure®Saphire Biomolecular Imager Azure Biosystems N/A CLARIOstar Plus with Atmospheric Control Unit (ACU) BMG LABTECH N/A Histopaque®-1077 Hybri-MaxTM Sigma-Aldrich Cat# H8889 Histopaque®-1119 Sigma-Aldrich Cat# 11191 iBLOT2 system Thermo Fisher Scientific (InvitrogenTM) N/A iBlotTM 2 Transfer Stacks, PVDF, mini Thermo Fisher Scientific (InvitrogenTM) Cat# IB24002 LumiCycle Actimetrics N/A NuPAGETM Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm Thermo Fisher Scientific (InvitrogenTM) Cat# NP0323BOX QuantStudio5TM Applied Biosystems N/A Radiance Q Chemiluminescent Substrate Azure Biosystems Cat# AC2101 SpectraMax® i3x Molecular Devices N/A iScience 28, 112431, May 16, 2025 e2 Pancreatic islets for circadian bioluminescence recordings were isolated from adult (approximately 15 weeks old) female C57BL/ 6J mice expressing the circadian reporter Per2-luciferase (mPer2:Luc) (The Jackson Laboratory, Bar Harbor, ME, USA).61 Mice were kept under standard temperature-controlled animal housing conditions with ad libitum access to food and water and 12h:12h lightdark cycle. ..
Control:Article Title: Circadian synchronization differentially modifies cytokine-mediated transcriptomic remodeling and cell death in INS-1 cells and mouse islets
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rat: INS-1 Gift from C. Wollheim and P. Maechler, University Medical Centre, Geneva, Switzerland RRID: CVCL_0352 Experimental models: Organisms/strains Mouse: C57Bl/6J Charles River Laboratories RRID: IMSR_CRL:027 Mouse: C57BL/6J mPer2:Luc The Jackson Laboratory RRID: IMSR_JAX:006852 Oligonucleotides For primers see Table S3 TAG Copenhagen N/A Recombinant DNA pPer2:Luc Gift from Prof. Louis Ptá�cek, University of California San Francisco N/A psPAX2 N/A RRID: Addgene_12260 pMD2.G N/A RRID: Addgene_12259 Software and algorithms AzureSpot Pro Azure Biosystems https://azurebiosystems.com/ software/azurespotpro/ BioDare2 Zieli�nski et al.59 https://biodare2.ed.ac.uk/ GraphPad Prism 9/10 GraphPad Software https://www.graphpad.com/ Image Lab 6.1 Bio-Rad Laboratories https://www.bio-rad.com/product/ image-lab-software?ID=KRE6P5E8Z Improved Proteasome Cleavage Prediction Server (iPCPS) Gomez-Perosanz et al.60 http://imed.med.ucm.es/Tools/pcps/ QuantStudioTM Design & Analysis Software Applied Biosystems https://www.thermofisher.com/home/ technical-resources/software-downloads/ quantstudio-3-5-real-time-pcr-systems.html Other Azure®Saphire Biomolecular Imager Azure Biosystems N/A CLARIOstar Plus with Atmospheric Control Unit (ACU) BMG LABTECH N/A Histopaque®-1077 Hybri-MaxTM Sigma-Aldrich Cat# H8889 Histopaque®-1119 Sigma-Aldrich Cat# 11191 iBLOT2 system Thermo Fisher Scientific (InvitrogenTM) N/A iBlotTM 2 Transfer Stacks, PVDF, mini Thermo Fisher Scientific (InvitrogenTM) Cat# IB24002 LumiCycle Actimetrics N/A NuPAGETM Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm Thermo Fisher Scientific (InvitrogenTM) Cat# NP0323BOX QuantStudio5TM Applied Biosystems N/A Radiance Q Chemiluminescent Substrate Azure Biosystems Cat# AC2101 SpectraMax® i3x Molecular Devices N/A iScience 28, 112431, May 16, 2025 e2 Pancreatic islets for circadian bioluminescence recordings were isolated from adult (approximately 15 weeks old) female C57BL/ 6J mice expressing the circadian reporter Per2-luciferase (mPer2:Luc) (The Jackson Laboratory, Bar Harbor, ME, USA).61 Mice were kept under standard temperature-controlled animal housing conditions with ad libitum access to food and water and 12h:12h lightdark cycle. ..
Isolation:Article Title: Circadian synchronization differentially modifies cytokine-mediated transcriptomic remodeling and cell death in INS-1 cells and mouse islets
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rat: INS-1 Gift from C. Wollheim and P. Maechler, University Medical Centre, Geneva, Switzerland RRID: CVCL_0352 Experimental models: Organisms/strains Mouse: C57Bl/6J Charles River Laboratories RRID: IMSR_CRL:027 Mouse: C57BL/6J mPer2:Luc The Jackson Laboratory RRID: IMSR_JAX:006852 Oligonucleotides For primers see Table S3 TAG Copenhagen N/A Recombinant DNA pPer2:Luc Gift from Prof. Louis Ptá�cek, University of California San Francisco N/A psPAX2 N/A RRID: Addgene_12260 pMD2.G N/A RRID: Addgene_12259 Software and algorithms AzureSpot Pro Azure Biosystems https://azurebiosystems.com/ software/azurespotpro/ BioDare2 Zieli�nski et al.59 https://biodare2.ed.ac.uk/ GraphPad Prism 9/10 GraphPad Software https://www.graphpad.com/ Image Lab 6.1 Bio-Rad Laboratories https://www.bio-rad.com/product/ image-lab-software?ID=KRE6P5E8Z Improved Proteasome Cleavage Prediction Server (iPCPS) Gomez-Perosanz et al.60 http://imed.med.ucm.es/Tools/pcps/ QuantStudioTM Design & Analysis Software Applied Biosystems https://www.thermofisher.com/home/ technical-resources/software-downloads/ quantstudio-3-5-real-time-pcr-systems.html Other Azure®Saphire Biomolecular Imager Azure Biosystems N/A CLARIOstar Plus with Atmospheric Control Unit (ACU) BMG LABTECH N/A Histopaque®-1077 Hybri-MaxTM Sigma-Aldrich Cat# H8889 Histopaque®-1119 Sigma-Aldrich Cat# 11191 iBLOT2 system Thermo Fisher Scientific (InvitrogenTM) N/A iBlotTM 2 Transfer Stacks, PVDF, mini Thermo Fisher Scientific (InvitrogenTM) Cat# IB24002 LumiCycle Actimetrics N/A NuPAGETM Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm Thermo Fisher Scientific (InvitrogenTM) Cat# NP0323BOX QuantStudio5TM Applied Biosystems N/A Radiance Q Chemiluminescent Substrate Azure Biosystems Cat# AC2101 SpectraMax® i3x Molecular Devices N/A iScience 28, 112431, May 16, 2025 e2 Pancreatic islets for circadian bioluminescence recordings were isolated from adult (approximately 15 weeks old) female C57BL/ 6J mice expressing the circadian reporter Per2-luciferase (mPer2:Luc) (The Jackson Laboratory, Bar Harbor, ME, USA).61 Mice were kept under standard temperature-controlled animal housing conditions with ad libitum access to food and water and 12h:12h lightdark cycle. ..
Expressing:Article Title: Circadian synchronization differentially modifies cytokine-mediated transcriptomic remodeling and cell death in INS-1 cells and mouse islets
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rat: INS-1 Gift from C. Wollheim and P. Maechler, University Medical Centre, Geneva, Switzerland RRID: CVCL_0352 Experimental models: Organisms/strains Mouse: C57Bl/6J Charles River Laboratories RRID: IMSR_CRL:027 Mouse: C57BL/6J mPer2:Luc The Jackson Laboratory RRID: IMSR_JAX:006852 Oligonucleotides For primers see Table S3 TAG Copenhagen N/A Recombinant DNA pPer2:Luc Gift from Prof. Louis Ptá�cek, University of California San Francisco N/A psPAX2 N/A RRID: Addgene_12260 pMD2.G N/A RRID: Addgene_12259 Software and algorithms AzureSpot Pro Azure Biosystems https://azurebiosystems.com/ software/azurespotpro/ BioDare2 Zieli�nski et al.59 https://biodare2.ed.ac.uk/ GraphPad Prism 9/10 GraphPad Software https://www.graphpad.com/ Image Lab 6.1 Bio-Rad Laboratories https://www.bio-rad.com/product/ image-lab-software?ID=KRE6P5E8Z Improved Proteasome Cleavage Prediction Server (iPCPS) Gomez-Perosanz et al.60 http://imed.med.ucm.es/Tools/pcps/ QuantStudioTM Design & Analysis Software Applied Biosystems https://www.thermofisher.com/home/ technical-resources/software-downloads/ quantstudio-3-5-real-time-pcr-systems.html Other Azure®Saphire Biomolecular Imager Azure Biosystems N/A CLARIOstar Plus with Atmospheric Control Unit (ACU) BMG LABTECH N/A Histopaque®-1077 Hybri-MaxTM Sigma-Aldrich Cat# H8889 Histopaque®-1119 Sigma-Aldrich Cat# 11191 iBLOT2 system Thermo Fisher Scientific (InvitrogenTM) N/A iBlotTM 2 Transfer Stacks, PVDF, mini Thermo Fisher Scientific (InvitrogenTM) Cat# IB24002 LumiCycle Actimetrics N/A NuPAGETM Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm Thermo Fisher Scientific (InvitrogenTM) Cat# NP0323BOX QuantStudio5TM Applied Biosystems N/A Radiance Q Chemiluminescent Substrate Azure Biosystems Cat# AC2101 SpectraMax® i3x Molecular Devices N/A iScience 28, 112431, May 16, 2025 e2 Pancreatic islets for circadian bioluminescence recordings were isolated from adult (approximately 15 weeks old) female C57BL/ 6J mice expressing the circadian reporter Per2-luciferase (mPer2:Luc) (The Jackson Laboratory, Bar Harbor, ME, USA).61 Mice were kept under standard temperature-controlled animal housing conditions with ad libitum access to food and water and 12h:12h lightdark cycle. ..
Mouse Assay:Article Title: Circadian synchronization differentially modifies cytokine-mediated transcriptomic remodeling and cell death in INS-1 cells and mouse islets
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rat: INS-1 Gift from C. Wollheim and P. Maechler, University Medical Centre, Geneva, Switzerland RRID: CVCL_0352 Experimental models: Organisms/strains Mouse: C57Bl/6J Charles River Laboratories RRID: IMSR_CRL:027 Mouse: C57BL/6J mPer2:Luc The Jackson Laboratory RRID: IMSR_JAX:006852 Oligonucleotides For primers see Table S3 TAG Copenhagen N/A Recombinant DNA pPer2:Luc Gift from Prof. Louis Ptá�cek, University of California San Francisco N/A psPAX2 N/A RRID: Addgene_12260 pMD2.G N/A RRID: Addgene_12259 Software and algorithms AzureSpot Pro Azure Biosystems https://azurebiosystems.com/ software/azurespotpro/ BioDare2 Zieli�nski et al.59 https://biodare2.ed.ac.uk/ GraphPad Prism 9/10 GraphPad Software https://www.graphpad.com/ Image Lab 6.1 Bio-Rad Laboratories https://www.bio-rad.com/product/ image-lab-software?ID=KRE6P5E8Z Improved Proteasome Cleavage Prediction Server (iPCPS) Gomez-Perosanz et al.60 http://imed.med.ucm.es/Tools/pcps/ QuantStudioTM Design & Analysis Software Applied Biosystems https://www.thermofisher.com/home/ technical-resources/software-downloads/ quantstudio-3-5-real-time-pcr-systems.html Other Azure®Saphire Biomolecular Imager Azure Biosystems N/A CLARIOstar Plus with Atmospheric Control Unit (ACU) BMG LABTECH N/A Histopaque®-1077 Hybri-MaxTM Sigma-Aldrich Cat# H8889 Histopaque®-1119 Sigma-Aldrich Cat# 11191 iBLOT2 system Thermo Fisher Scientific (InvitrogenTM) N/A iBlotTM 2 Transfer Stacks, PVDF, mini Thermo Fisher Scientific (InvitrogenTM) Cat# IB24002 LumiCycle Actimetrics N/A NuPAGETM Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm Thermo Fisher Scientific (InvitrogenTM) Cat# NP0323BOX QuantStudio5TM Applied Biosystems N/A Radiance Q Chemiluminescent Substrate Azure Biosystems Cat# AC2101 SpectraMax® i3x Molecular Devices N/A iScience 28, 112431, May 16, 2025 e2 Pancreatic islets for circadian bioluminescence recordings were isolated from adult (approximately 15 weeks old) female C57BL/ 6J mice expressing the circadian reporter Per2-luciferase (mPer2:Luc) (The Jackson Laboratory, Bar Harbor, ME, USA).61 Mice were kept under standard temperature-controlled animal housing conditions with ad libitum access to food and water and 12h:12h lightdark cycle. ..
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